Thursday, September 1, 2011

A Novel Autologous Scaffold for Diced-cartilage Grafts in Dorsal Augmentation Rhinoplasty.

A Novel Autologous Scaffold for Diced-cartilage Grafts in Dorsal Augmentation Rhinoplasty.

Aesthetic Plast Surg. 2011 Aug;35(4):569-79

Authors: Bullocks JM, Echo A, Guerra G, Stal S, Yuksel E

Abstract
BACKGROUND: Diced-cartilage grafts have been used for dorsal nasal augmentation for several years with good results. However, compounds such as Surgicel and temporalis fascia used as a wrap have inherent problems associated with them, predominantly inflammation and graft resorption. An autologous carrier could provide stabilization of cartilage grafts while avoiding the complications seen with earlier techniques.
METHODS: In our patients, a malleable construct was used for dorsal nasal augmentation in which autologous diced-cartilage grafts were stabilized with autologous tissue glue (ATG) created from platelet-rich plasma (platelet gel) and platelet-poor plasma (fibrin glue).
RESULTS: A prospective analysis of 68 patients, who underwent dorsal nasal augmentation utilizing ATG and diced-cartilage grafts between 2005 and 2008, were included in the study. Although there was notable maintenance of the dorsal height, no complications occurred that required explantation over a mean follow-up of 15�months.
CONCLUSION: The use of ATG to stabilize diced-cartilage grafts is a safe, reliable technique for dorsal nasal augmentation. The platelet gel provides growth factors while the fibrin glue creates a scaffold that allows stabilization and diffusion of nutrients to the cartilage graft.

PMID: 21487909 [PubMed - in process]

Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=21487909&dopt=Abstract

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Micro- and Macroscopic Structural Modification of Subcutaneous Adipose Tissue After Bariatric Surgery.

Micro- and Macroscopic Structural Modification of Subcutaneous Adipose Tissue After Bariatric Surgery.

Aesthetic Plast Surg. 2011 Jun 8;

Authors: D'Ettorre M, Gniuli D, Bracaglia R, Tambasco D, Mingrone G, Gentileschi S, Massi G

PMID: 21656061 [PubMed - as supplied by publisher]

Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=21656061&dopt=Abstract

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Information on the new procedure "Buttocks Augmentation" is now available at PlasticSurgery.com

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Source: http://www.plasticsurgery.com/procedure-Buttocks_Augmentation/procedure/info.html

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Information on the new procedure "Breast Augmentation" is now available at PlasticSurgery.com

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Stem Cells, Mature Adipocytes, and Extracellular Scaffold: What Does Each Contribute to Fat Graft Survival?

Stem Cells, Mature Adipocytes, and Extracellular Scaffold: What Does Each Contribute to Fat Graft Survival?

Aesthetic Plast Surg. 2011 May 18;

Authors: Mojallal A, Lequeux C, Shipkov C, Rifkin L, Rohrich R, Duclos A, Brown S, Damour O

Abstract
BACKGROUND: Soft tissue engineering offers new perspectives for improving fat graft survival, for which the appropriate association of cells and scaffold seems essential. This study aimed to analyze the survival of free-cell grafts compared with adipose-derived stem cells (ASCs) seeded on collagen scaffolds. METHODS: Adipose tissue from a single volunteer was used for the following preparations: purified adipose tissue, isolated mature adipocytes (free-cell graft), cultured ASCs without scaffold (free-cell graft), collagen scaffold only, cultured ASCs in collagen scaffold without and with bioactive factors, and freshly-isolated ASCs in collagen scaffold. These were grafted on 18 nude mice for 2�months, after which specimens were evaluated grossly and histologically using hematoxylin-phloxine-safran (HPS), Oil-Red-O, and antivimentin labeling. Specimens and animals were weighed before implantation and after explantation, and weight values were statistically analyzed. RESULTS: Free-cell grafts (mature adipocytes and free ASCs) showed complete resorption in 50 and 60% of the animals (remaining weight fraction was 22.5 and 5.3%, respectively). The survival of purified adipose tissue was 81.8% (statistically greater compared with free-cell grafts; p�<�0.05). In the ASCs-scaffold association, the remaining weight fractions (87.3-70.4%) were statistically greater than in free-cell grafts (5.3-22.5%; p�<�0.05), but the difference between ASC-scaffolds and fat grafts was not statistically significant. These results were confirmed by clinical and histologic observations. CONCLUSION: Three-dimensional collagen scaffolds seem to improve survival of ASCs compared with free-cell grafts (adipocytes and free ASCs).

PMID: 21590499 [PubMed - as supplied by publisher]

Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=21590499&dopt=Abstract

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