In Vitro N-Acetyl-L: -Cysteine Promotes Proliferation and Suppresses Interleukin-8 Expression in Adipose-Derived Stem Cells.
Aesthetic Plast Surg. 2012 Aug 31;
Authors: Xiong L, Sun J, Hirche C, Yang J, Yang Y, Xia Y, Lehnhardt M, Wang R, Fu X
Abstract
Adipose-derived stem cells (ADSCs) hold great promise for repair and regeneration of burn wounds by producing growth factors, but proinflammatory cytokines such as interleukin-8 (IL-8) released by ADSCs would potentially deepen the wound and inhibit healing. The reported research aimed to identify the effects of N-acetyl-L: -cysteine (NAC) on the proliferation, death, and IL-8 production of ADSCs. In the presence or absence of NAC, ADSC proliferation was examined using a CCK-8 Kit, and cell death was evaluated by flow cytometry analysis. Subsequently, IL-8 mRNA expression was detected by reverse transcriptase-polymerase chain reaction and protein production by enzyme-linked immunoassay. Findings showed that cell proliferation in the NAC-treated group was a significant 1.53-fold greater than in the control group, that the apoptosis rate of ADSCs decreased by 55.4�% compared with the control group, and that the necrosis rate decreased by 48.8�%. Additionally, the IL-8 mRNA expression decreased to 46.2���8.7�% that of the control group, and the IL-8 protein production decreased to 9.98���0.57�%. The authors believe that NAC might be helpful in burn wound repair and regeneration by stimulating the proliferation of ADSCs, inhibiting cell death, and suppressing IL-8 production. LEVEL OF EVIDENCE II: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
PMID: 22936379 [PubMed - as supplied by publisher]
Source: http://www.ncbi.nlm.nih.gov/PubMed/22936379?dopt=Abstract
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