Establishing efficient xenograft models with intrinsic vascularisation for growing primary human low-grade sarcomas.

Establishing efficient xenograft models with intrinsic vascularisation for growing primary human low-grade sarcomas.

Anticancer Res. 2011 Dec;31(12):4061-6

Authors: Tilkorn D, Daigeler A, Stricker I, Schaffran A, Schmitz I, Steinstraesser L, Hauser J, Ring A, Steinau HU, Al-Benna S

Abstract
BACKGROUND: There are no xenograft models of low-grade soft tissue sarcoma. Transplant survival remains an obstacle in sarcoma xenograft models and is attributed to post-transplantation hypoxia. Models with an intrinsic tissue - engineered vascular supply may overcome this obstacle. The aim of this study was to establish a novel xenograft model of primary human low grade soft tissue sarcoma.
MATERIALS AND METHODS: Primary low-grade liposarcoma fragments were transplanted into a silicon chamber, placed around the superficial epigastric vessels in athymic nude mice. Xenograft samples were assessed histologically (light/electron microscopy and immunohistochemistry for S100).
RESULTS: All xenotransplants of low grade primary soft tissue liposarcoma (n=4) engrafted, led to the development of solid tumours in mice. Histological and immunohistochemical staining confirmed the xenografts as being well-differentiated liposarcomas identical to the original tumor tissue.
CONCLUSION: Successful transplantation of human low-grade liposarcoma tissue in mice was established for the first time using a model with an intrinsic vascular supply.

PMID: 22199262 [PubMed - indexed for MEDLINE]

Source: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=22199262&dopt=Abstract

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